Rapid dehydration--clearing with 2,2-dimethoxypropane for paraffin embedding.

A Rapid Method of Paraffin Embedding

Preparation of serial sections of arthropods using 2,2-dimethoxypropane dehydration and epoxy resin embedding under vacuum.

Improved methods are described for anatomical investigation of small insects and other arthropods using serial semithin sections. The specimens were dehydrated with acidified 2,2-dimethoxypropane and embedded in ERL 4206 epoxy resin under vacuum. This procedure ensures good resin impregnation of thin, long body compartments and appendages. Furthermore, it produces excellent overall preservation...

Immunostaining, dehydration, and clearing of mouse embryos for ultramicroscopy.

This protocol describes the preparation of mouse embryos for ultramicroscopy (UM), a powerful imaging technique that achieves precise and accurate three-dimensional (3D) reconstructions of intact macroscopic specimens with micrometer resolution. In UM, a specimen in the size range of ∼1-15 mm is illuminated perpendicular to the observation pathway by two thin counterpropagating sheets of laser ...

nylon-mesh bag or a plastic tissue-basket. Dehydration with alcohol and celloidin embedding: Dehydration

Precise knowledge of various ganglia is very important for a' thorough understanding of clinical medicine. Details of these ganglia, however, have not been included in textbook descriptions. We conducted our research to bring precise figures to light. We selected several ganglia as follows: the GG, which is the site of nerve cell bodies for the special sense of taste; the SG, which supplies pre...

Agar-gelatin for embedding tissues prior to paraffin processing.

Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...